In-Brief: Applying Internal Validation Guidelines for Expanded STR Loci Kits

In-Brief: Applying Internal Validation Guidelines for Expanded STR Loci Kits


January 2017


The familiar ‘13 Combined DNA Index System (CODIS) core loci’ expanded to ‘20 CODIS core loci’ as mandated by the FBI on January 1, 2017. Because of this FBI mandate, participating CODIS laboratories will need to internally validate an expanded STR loci kit for implementation in 2017. This FTCOE validation in-brief serves to provide an overview of already existing guidance from the Scientific Working Group on DNA Analysis Methods (SWGDAM) and the Organization of Scientific Area Committees (OSAC) on validation requirements and recommendations.

“The new expansion includes loci aimed to become more compatible with international databases, increase the power of discrimination, and reduce the opportunity for accidental matches as the CODIS database expands in size.”

- Douglas R. Hares

Funding for this Forensic Technology Center of Excellence report was provided by the National Institute of Justice, Office of Justice Programs, U.S. Department of Justice.

The opinions, findings, and conclusions or recommendations expressed in this report are those of the author(s) and do not necessarily reflect those of the U.S. Department of Justice.

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