Microhaplotypes: A Comprehensive Forensic DNA Marker

Microhaplotypes: A Comprehensive Forensic DNA Marker

This webinar originally occurred on June 22nd, 2021
Duration: 1 hour

In this presentation, the subject matter expert discussed the current status of the research conducted in the GW-FMB lab on a new multi-function DNA marker and its potential impact to the field of forensic genetics.  

Microhaplotypes (microhaps) are biomarkers less than 300 nucleotides long that display multiple allelic combinations. The main advantages of microhaps over conventional short tandem repeats (STRs) include the 1) absence of stutter, 2) same-size alleles within each locus, 3) lower mutation rate, and 4) ancestry informative alleles. These forensically relevant loci can yield a power of discrimination similar to STRs while enhancing human identification (HID), mixture deconvolution, and biogeographic ancestry prediction.  

 Sanger sequencing does not allow determining the cis/trans relationship among closely related SNPs, however massively parallel sequencing (MPS) allows determining the parental haplotypes at each locus by clonally sequencing of each DNA molecule if they are included in the same amplicon. Currently, STR panels are used for mixture deconvolution and SNP assays are used for ancestry inference. Microhaplotypes can be used for both functions allowing, for example, to infer the ancestry of a minor contributor to a mixture (DNA intelligence).  

Details Learning Objectives

Attendees will learn about:

  1. Microhaplotypes (MHs) as an emerging forensic marker
  2. Massively parallel sequencing (MPS) methods applied to forensic DNA analysis
  3. Adapting probabilistic genotyping to data from MPS of MHs



Daniele Podini -Professor in the Department of Forensic Sciences at George Washington University


Funding for this Forensic Technology Center of Excellence event has been provided by the National Institute of Justice.-

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