Forensic Technology Center of Excellence

Biology & DNA

Evidence Collection and Analysis for Touch Deoxyribonucleic Acid in Groping and Sexual Assault Cases

Report Publication Journal of Forensic Nursing, April 2021 Introduction Historically, evidence collection in sexual assault cases focused on obtaining foreign contributor bodily fluids through swab collection. With improvements in deoxyribonucleic acid (DNA) analysis methods, DNA profiles can be developed from touch…
DNA strands

Perspectives on Addressing the Collection, Tracking, and Processing of Lawfully Owed DNA Samples

Report Date April 2021 Introduction The Federal Bureau of Investigation’s (FBI’s) Combined DNA Index System (CODIS) is a powerful tool that supports the criminal justice system by generating investigative leads, identifying perpetrators, and linking cases together. DNA profiles obtained from…

Introduction to Touch DNA Evidence for Sexual Assault Groping Cases Video Series

Overview: The Importance of Touch DNA Evidence in Sexual Assault Groping Cases video series is presented by Dr. Julie Valentine and Heather Mills as a companion to the Journal of Forensic Nursing article entitled “Evidence Collection and Analysis for Touch…
constellations over glass lab equipment

Forensic Laboratory Needs Technical Working Group

Supporting NIJ’s Mission to Improve Knowledge and Understanding The National Institute of Justice (NIJ), in partnership with the Forensic Technology Center of Excellence (FTCoE) at RTI International (Research Triangle Institute), has formed the Forensic Laboratory Needs – Technology Working Group…

Stability and Persistence of Touch DNA for Forensic Analysis

Touch DNA evidence (i.e. the low quantities of DNA that can be left behind when someone touches an object) is of increasing importance in criminal investigations. However, many important questions exist regarding basic properties of touch DNA, including the expected…
a digital strand of DNA

An Automated Single Cell Separation Technique to Improve Mixture Deconvolution

Thursday, November 12th, 2020 1:00:00 PM ET – 2:00:00 PM ET Duration: 1 hour Although the interpretation of mixed DNA samples is commonplace in forensic analyses, complex samples often present interpretational challenges. These complexities are introduced through a variety of…
microphone and headphones

Just Forensic Genetic Genealogy and GEDmatch:  Verogen’s Approach

In this Forensic Science Week special episode, Just Science interviews Brett Williams, the CEO of Verogen, about the GEDmatch database, how it can be used by law enforcement to perform forensic genealogy searches for investigative leads, and the short- and long-term plans Verogen has for…
image of DNA

PACE™: Rapid and Automated Artifact Identification and Number of Contributor Prediction

– Tuesday, July 28th, 2020 1:00:00 PM ET – 2:00:00 PM ETDuration: 1 hour(s) The PACE™ software introduces a fully continuous, machine learning tool to the forensic DNA community that enables fast and accurate artifact management and predicts the number…
virtual depiction of DNA strands on computer screen

Validation and Tutorial of NOCIt for Determining the Number of Contributors

– Tuesday, July 14th, 2020 1:00:00 PM ET – 2:00:00 PM ET Duration: 1 hour(s) Forensic DNA signal is notoriously challenging to interpret and requires the implementation of computational tools that support its interpretation. While data from high-copy, low-contributor samples…

2019 National Institute of Justice Forensic Science Research and Development Symposium

This report was published in RTI Press, a global publisher of peer-reviewed, open-access publications on a broad range of topics. The areas of focus reflect RTI’s multidisciplinary research, our expertise in social and laboratory sciences, and our extensive international activities. Since…
eye dropper filling glass test tubes

The Time to Analyze is Now: DNA Evidence in Groping Sexual Assault Cases

Wednesday, January 29th, 2020 1:00:00 PM ET – 2:30:00 PM ET Duration: 1.5 hour(s) This webinar built on the concepts presented in the webinar “The Time to Collect is Now: DNA Evidence in Groping Sexual Assault Cases” presented by Dr.…

DNA Backlog Reduction Strategy: Law Enforcement Agency Partnerships for a Successful Biological Screening Laboratory

NIJ’s FTCoE, Palm Beach County Sheriff’s Department, and Boca Raton Police Services Department   Report Date November 2019 Introduction Implementing DNA technology in crime laboratories has led to the intensification of backlogged cases and a concomitant pressure to implement backlog…
virtual depiction of DNA strands

ASCLD Train the Director Series – Investigative Genetic Genealogy: Background and Crime Lab Strategy

← Back to Series  Thursday, January 23rd, 2020 1:00:00 PM ET – 2:30:00 PM ET Duration: 1.5 hour(s) Investigative Genetic Genealogy is a recently developed technique that generates new leads on previously unsolved cases where DNA from the suspect is…

Online Workshop Series: Probabilistic Genotyping Of Evidentiary DNA Typing Results

Overview The Federal Bureau of Investigation Laboratory has partnered with the National Institute of Justice’s Forensic Technology Center of Excellence to create a series of online workshops aimed at increasing knowledge, understanding, and the reliable application of probabilistic genotyping to…

Success Story: Enabling Forensic Field Testing with Paper Microfluidic Devices

NIJ and Florida International University Report Date April 2019 Impact “I’m impressed with the potential for replacement wet color tests. The multiplexing capabilities have potential to address the challenges field forensics investigators encounter with non-pure, intermixed drugs as well as…

Success Story: Improving DNA Mixture Interpretation with the Help of Machine Learning

NIJ and Syracuse University Report Date March 2019 Impact “This is something that every DNA crime lab worldwide should investigate. We now have a tool that with the press of one button does the entire suite of services and provides…

The Time to Collect is Now: DNA Evidence in Groping Sexual Assault Cases

Wednesday, February 27, 2019 2:00 PM ET – 3:30 PM ET Duration: 1.5 hour(s) Improving the overall response to sexual assault includes understanding how touch DNA evidence impacts groping sexual assault cases. This webinar will illustrate the importance of these…

In-Brief Report Series: Beyond DNA – Sexual Assault Investigations

Report Series Date January 2019 Introduction The National Institute of Justice (NIJ) is committed in their efforts to support scientific advancement, evidence-based practices, and community awareness of our Nation’s sexual assault response. As NIJ’s Director, Dr. David B. Muhlhausen, indicated,…

Success Story: Uncovering Key Details of Skeletal Remains through 3D-ID Software

NIJ and North Carolina State University Report Date January 2019 Impact “3D-ID has been an invaluable tool for us. Many of our unknown persons come from populations south of the Border. We use this program for every case with great…

Process and Outcome Evaluation of Forensic DNA Unit Efficiency Improvement Program

Technical Note Date November 2018 Description A 2009 National Institute of Justice (NIJ) Forensic DNA Unit Efficiency Improvement (EIP) Program solicitation provided crime laboratories’ DNA testing services with a funding opportunity to meet the increasingly numerous requests from the criminal…

A Landscape Study of SOPs to Collect and Process Arrestee and Convicted Offender DNA Samples

Report Date This study is currently being conducted. The project team presented a poster on preliminary findings and next steps at the International Symposium on Human Identification (ISHI) in Phoenix, Arizona on September 24-27, 2018. Description The Forensic Technology Center…

Advancing Research Initiatives and Combatting the Human Trafficking Epidemic

Thursday, November 1, 2018 1:00 PM ET – 2:00 PM ET Duration: 1 hour(s) Trafficking in persons is a multi-faceted global epidemic which has seen a significant increase in public awareness. However, there is a substantial need to expand capacities…

Estimating Blow Fly Age and Reducing Error in Postmortem Interval Cases

Wednesday, October 24, 2018 2:00:00 PM ET – 4:00:00 PM ET Duration: 2 hour(s) Insect evidence can provide valuable information during death investigations. Forensic entomologists estimate the ages of the immature insects like blow flies, which can provide useful information…

New SWGDAM Recommendations on Communicating Likelihood Ratios

Thursday, October 18, 2018 1:00:00 PM ET – 3:00:00 PM ET Duration: 2 hour(s) SWGDAM recently issued recommendations on genotyping results reported as likelihood ratios, designed to promote consistency among laboratories. Members of the committee will discuss the recommendations and…

Mixtures, Microhaps, and Modeling, Oh My!! Cutting-Edge Research in Forensic DNA Analysis from Early-Career Scientists

Tuesday, September 18, 2018 10:00:00 AM ET – 11:30:00 AM ET Duration: 1.5 hour(s) During National Forensic Science Week, early-career researchers that were awarded FTCoE travel sponsorships to the prestigious Gordon Research Conference and Seminar will present novel projects in…

Conference Proceedings: 2018 Research and Development Symposium

This report was published in RTI Press, a global publisher of peer-reviewed, open-access publications on a broad range of topics. The areas of focus reflect RTI’s multidisciplinary research, our expertise in social and laboratory sciences, and our extensive international activities. Since…

Success Story: Establishing a “Microbial Clock” to Improve Time of Death Prediction

NIJ and Multi-Institute Academic Team Report Date April 2018 Impact “This research clearly demonstrated that there is potential to develop microbiome tools for estimating time of death. Developing connections with the right interdisciplinary collaborators was instrumental to the success of…

Success Story: Maximizing the Use of Mitochondrial DNA in Identifying Remains and Aiding Missing Persons Casework

NIJ and the American Registry of Pathology Report Date April 2018 Impact “The complete mtGenome reference data developed in this project serve as important milestones for the understanding of coding region heteroplasmy and the acceptance of full mtGenome sequencing in…

Success Story: NIST’s Dogs and Sensor Subcommittee Builds on Achievements by SWGDOG

NIJ and Florida International University Report Date April 2018 Impact “SWGDOG set the stage and provided a strong foundation of canine detector best practices that the OSAC Dogs and Sensor Subcommittee is currently developing into scientifically robust standards. The Subcommittee…

Evaluate Population-Specific Sibship During Kinship Analysis

← Back to Series  – This webinar will provide an overview of what kinship analysis programs are currently in use and will provide an in-depth analysis of how population-based allele frequency databases impact sibship determination. Kinship analysis of short tandem…

Evaluating the Use of DNA Analysis for Family Reunification

← Back to Series  DNA analysis allows for testing relationships (kinship) between two individuals in situations that vary from legal immigration to claimed parentage testing. This webinar focuses on the applicability of DNA testing for family reunification. Biometrics refers to…

Cost-Benefit Analysis of Kinship Testing Involving Siblings

← Back to Series  This webinar aims to improve the knowledge on kinship testing involving siblings and provides a cost-benefit analysis of practices involving the use of additional reference siblings and substitution of half-siblings for full siblings in kinship tests.…
DNA strand

Success Story: Forensic Epigenetics – Markers for the Identification of Body Fluids

NIJ and Florida International University Report Date February 2018 Impact “Our laboratory is excited about DNA epigenetic markers as they offer the possibility of an objective confirmation of what cell type the DNA profile is originating from. If proven, the…

Predict Human Appearance From DNA Focusing On Pigmentation

Wednesday, October 25, 2017 10:00:00 AM EDT – 12:00:00 PM EDT Duration: 2 hour(s) Principles, methods and applications of DNA-based prediction of human appearance traits for forensic and anthropological use with specific insights into eye, hair, and skin colour prediction…

2016 National Institute of Justice Forensic Science Research and Development Symposium

This report was published in RTI Press, a global publisher of peer-reviewed, open-access publications on a broad range of topics. The areas of focus reflect RTI’s multidisciplinary research, our expertise in social and laboratory sciences, and our extensive international activities. Since…

ASCLD Webinar Series: DNA Standards and Guidelines Part 1

This webinar was targeted to DNA Supervisors, DNA Technical Leaders, Quality Mangers, and DNA Analysts. There was also a question and answer period after the presentation. Forensic DNA Analysis, and in particular interpreting complex DNA mixtures and calculating statistics, can…

In-Brief: Applying Internal Validation Guidelines for Expanded STR Loci Kits

Report Date January 2017 Report Summary The familiar ‘13 Combined DNA Index System (CODIS) core loci’ expanded to ‘20 CODIS core loci’ as mandated by the FBI on January 1, 2017. Because of this FBI mandate, participating CODIS laboratories will need…

A Guidance Document on the Forensic Application of Massively Parallel Sequencing

Report Date December 2016 Purpose This report describes the fundamentals of massively parallel sequencing (MPS) for the application to forensic analysis. Also included within this report is a comprehensive summary of the webinar series which discussed the strength of the technology…

Genome ID Forum 2014 – The Critical Role of SNPs in the Future of Forensics

Originally presented at the 2014 Genome ID Forum, this presentation appraises the potential value of Single Nucleotide Polymorphisms (SNPs) as applied to forensic analysis, including ancestry inference, phenotypic inference and individual identification. The discussion focuses strongly on the relevance of…

Genome ID Forum 2014 – Proteomics: Multiplex Assay to Identify Biological Stains

Originally presented at the 2014 Genome ID Forum, this archived presentation discusses the development and validation of a rapid multiplex assay for the identification of biological stains using comparative proteomics. Current serological techniques, although historically utilized and relied upon heavily…

Genome ID Forum 2014 – Legal Perspective on the Adoption of Advanced Technologies

Originally presented at the 2014 Genome ID Forum, Ted Hunt, chief trial attorney in the prosecuting attorney’s office in Kansas City, Missouri, brings a fresh legal perspective to addressing the adoption of advanced DNA analysis technologies for forensic crime laboratories. In…

Genome ID Forum 2014 – HD SNP Array for Forensic Casework

Originally presented at the 2014 Genome ID Forum, Dr. Sandra Close presents her latest research, the application of high density SNP array technology applied to forensic casework. Dr. Close presents an overview of the platform, including chemistry, assay and interpretation,…

Familial DNA Searching: Current Approaches – Part IV

This four-part panel discussion series will elucidate the current landscape of policies and procedures addressing familial DNA searching. Part IV In this particular session, the discussion focused around current technical protocols including software considerations, candidate thresholds derived by likelihood ratio…

Familial DNA Searching: Current Approaches – Part III

This four-part panel discussion series will elucidate the current landscape of policies and procedures addressing familial DNA searching. Part III In this particular session, the discussion focused around the process of investigative follow up from these leads including costs and…

Familial DNA Searching: Current Approaches – Part II

This four-part panel discussion series will elucidate the current landscape of policies and procedures addressing familial DNA searching. Part II In this particular session, the discussion topic was focused around current existing protocols and specifically address questions such as: what…

Familial DNA Searching: Current Approaches – Part I

This four-part panel discussion series will elucidate the current landscape of policies and procedures addressing familial DNA searching. Part I These discussions were used to generate a report designed to educate legal professionals, policy makers, law enforcement and forensic laboratory…

Genome ID Forum 2014 – Emerging Technologies for Human Remains Identification

Originally presented at the 2014 Genome ID Forum, an overview of the Laboratory’s new Next Generation Sequencing (NGS) instrumentation and protocol development is discussed including experimental design utilizing multiple forensic markers and the impact on sample processing. Funding for this…

ASCLD Webinar Series: FBI Allele Frequency – Managers & Legal Overview

Crime Laboratories have earned a position of public trust over time through providing reliable quality results. Keeping public trust includes being self-correcting once an issue is discovered. The Federal Bureau of Investigation allele frequency amendments is an opportunity to demonstrate…

ASCLD Webinar Series: FBI Allele Frequency Amendments – Technical Discussion

Crime Laboratories have earned a position of public trust over time through providing reliable quality results. Keeping public trust includes being self-correcting once an issue is discovered. The Federal Bureau of Investigation allele frequency amendments is an opportunity to demonstrate…

Genome ID Forum 2014 – Applications of Emerging Technologies NIST

Originally presented at the 2014 Genome ID Forum, the National Institute of Standards and Technology (NIST) SRMs includes the PCR based DNA profiling standard which covers 51 autosomal and 17 Y chromosome markers, mitochondrial DNA sequencing standards and the human…

A Landscape Study of DNA Mixture Interpretation Software

Report Date October 2015 Purpose Practical applications and implementation strategies for adoption of a DNA mixture interpretation software tool to assist with the challenges of complex mixture interpretation and statistical analysis. Report Summary Due to the influx of complex mixture…
skeletons

Success Story: Creating an International Databank of Skeletal Biomarkers for Human Identification

NIJ and University of South Florida Report Date September 2016 Impact “Forensic anthropology today is largely about applying methods and theory from studies in human variation to aid in the identification of human remains. The key is to find an…
mitochondria and DNA strands

Success Story: Customizing NextGENe Software for Forensic Applications

NIJ and CHORI – Collaboration with SoftGenetics® and California Department of Justice Report Date April 2016 Impact “Mitochondrial analysis holds significant potential for the forensic community and represents a great market opportunity for SoftGenetics®. As part of Dr. Calloway’s NIJ…
DNA strand gloved hand with tube

Success Story: Addressing a Key Challenge for PCR-Based Forensic Tests

NIJ and DNA Polymerase Technologies Report Date April 2016 Impact “DNA Polymerase Technologies has done impressive work engineering Taq mutants and in determining the proper combination of PCR enhancer cocktails to assess STR profiles in challenging samples.” – Robert Bever,…
skull with leaves and microbes

Success Story: Understanding the Ecology of Human Decomposition Methods for Estimating Postmortem Interval

NIJ and the National Museum of Health and Medicine Report Date April 2015 Impact “In life and death the body is its own ecosystem.” – Franklin Damann, PhD National Museum of Health and Medicine Click here to read the full…
DNA strand in magnifying glass and handcuffed hands

Success Story: Bringing Short Tandem Repeat DNA Identification to Law Enforcement

NIJ, UC Berkeley, and IntegenX Report Date April 2015 Impact “Ultimately, I think the most powerful application of this technology is going to be performing immediate analysis on DNA samples that are taken when people are arrested for felonies, because…