Forensic Technology Center of Excellence

Introduction

FLN-TWG: Proteomic Mass Spectrometry for Biology Fluid Identification

FLN-TWG: Proteomic Mass Spectrometry for Biology Fluid Identification

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Forensic Laboratory Needs Technology Working Group (FLN-TWG)

The National Institute of Justice (NIJ), in partnership with the Forensic Technology Center of Excellence (FTCoE) at RTI International, formed the Forensic Laboratory Needs Technology Working Group (FLN-TWG). The FLN-TWG supports NIJ’s mission to improve knowledge and understanding of the forensic technology needs of federal, state, local, and tribal forensic practitioners and crime laboratories.

Implementation Strategies: Proteomic Mass Spectrometry for Biology Fluid Identification

Report Date

February 2021

Overview

The proteomic mass spectrometry (MS) body fluid assay described herein is undergoing validation at the New York City Office of Chief Medical Examiner (NYC OCME). It is designed to detect multiple blood, saliva, and semen marker peptides using high performance liquid chromatography (HPLC) followed by multiple reaction monitoring (MRM) MS (employing an AB SCIEX Eksigent HPLC and 6500 QTRAP mass spectrometer respectively). The assay is unbiased, meaning no a priori knowledge of a sample is necessary before testing, because each sample is tested for all marker proteins. The assay is designed for high-throughput sample processing (~72 samples/day). On a volume basis, detection of each body fluid is in the nanoliter range (for both neat and mock forensic samples). According to Yang et al., sensitivity is at least 10 times greater than other presumptive or confirmatory methods, including chemical, immunochemical, enzymatic, and messenger RNA (mRNA) profiling.

Read Report Here


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